Pretreated biomass can directly be converted to ethanol by using the process called simultaneous saccharification and cofermentation (SSCF). Pretreatment is a critical step which enhances the enzymatic hydrolysis of biomass.

Basically, it alters the physical and chemical properties of biomass and improves the enzyme access and effectiveness which may also lead to a change in crystallinity and degree of polymerization of cellulose. The internal surface area and pore volume of pretreated biomass are increased which facilitates substantial improvement in accessibility of enzymes. The process also helps in enhancing the rate and yield of monomeric sugars during enzymatic hydrolysis steps.

Pretreatment methods can be broadly classified into four groups – physical, chemical, physio-chemical and biological. Physical pretreatment processes employ the mechanical comminution or irradiation processes to change only the physical characteristics of biomass. The physio-chemical process utilizes steam or steam and gases, like SO2 and CO2. The chemical processes employs acids (H2SO4, HCl, organic acids etc) or alkalis (NaOH, Na2CO3, Ca(OH)2, NH3 etc).

The acid treatment typically shows the selectivity towards hydrolyzing the hemicelluloses components, whereas alkalis have better selectivity for the lignin. The fractionation of biomass components after such processes help in improving the enzymes accessibility which is also important to the efficient utilization of enzymes.

The pretreated biomass is subjected to enzymatic hydrolysis using cellulase enzymes to convert the cellulose to fermentable sugars. Cellulase refers to a class of enzymes produced chiefly by fungi and bacteria which catalyzes the hydrolysis of cellulose by attacking the glycosidic linkages. Cellulase is mixture of mainly three different functional protein groups: exo-glucanase (Exo-G), endo-glucanase(Endo-G) and ?-glucosidase (?-G).

The functional proteins work synergistically in hydrolyzing the cellulose into the glucose. These sugars are further fermented using microorganism and are converted to ethanol. The microorganisms are selected based on their efficiency for ethanol productivity and higher product and inhibitors tolerance. Yeast Saccharomyces cerevisiae is used commercially to produce the ethanol from starch and sucrose.

Escherichia coli strain has also been developed recently for ethanol production by the first successful application of metabolic engineering. E. coli can consume variety of sugars and does not require the complex growth media but has very narrow operable range of pH. E. coli has higher optimal temperature than other known strains of bacteria.

The major cost components in bioethanol production from lignocellulosic biomass are the pretreatment and the enzymatic hydrolysis steps. In fact, these two process are someway interrelated too where an efficient pretreatment strategy can save substantial enzyme consumption.

Pretreatment step can also affect the cost of other operations such as size reduction prior to pretreatment. Therefore, optimization of these two important steps, which collectively contributes about 70% of the total processing cost, are the major challenges in the commercialization of bioethanol from 2nd generation feedstock.

Enzyme cost is the prime concern in full scale commercialization. The trend in enzyme cost is encouraging because of enormous research focus in this area and the cost is expected to go downward in future, which will make bioethanol an attractive option considering the benefits derived its lower greenhouse gas emissions and the empowerment of rural economy.

The post Biochemical Method for Ethanol Production first appeared on BioEnergy Consult.

Egypt is the world’s largest date producer with annual production of 1.47 million tons of dates in 2012 which accounted for almost one-fifth of global production. Saudi Arabia has more than 23 millions date palm trees, which produce about 1 million tons of dates per year.

Biomass Potential of Date Palm Wastes

Date palm trees produce huge amount of agricultural wastes in the form of dry leaves, stems, pits, seeds etc. A typical date tree can generate as much as 20 kilograms of dry leaves per annum while date pits account for almost 10 percent of date fruits. Some studies have reported that Saudi Arabia alone generates more than 200,000 tons of date palm biomass each year.

Date palm is considered a renewable natural resource because it can be replaced in a relatively short period of time. It takes 4 to 8 years for date palms to bear fruit after planting, and 7 to 10 years to produce viable yields for commercial harvest. Usually date palm wastes are burned in farms or disposed in landfills which cause environmental pollution in dates-producing nations. In countries like Iraq and Egypt, a small portion of palm biomass in used in making animal feed.

The major constituents of date palm biomass are cellulose, hemicelluloses and lignin. In addition, date palm has high volatile solids content and low moisture content. These factors make date biomass an excellent waste-to-energy resource in the MENA region.

Technology Options for Date Palm Biomass Utilization

A wide range of thermal and biochemical technologies exists to tap the energy stored in date palm biomass to useful forms of energy. The low moisture content in date palm wastes makes it well-suited to thermochemical conversion technologies like combustion, gasification and pyrolysis which may yield steam, syngas, bio oil etc.

On the other hand, the high volatile solids content in date palm biomass indicates its potential towards biogas production in anaerobic digestion plants, possibly by codigestion with sewage sludge, animal wastes and/and food wastes. The cellulosic content in date palm wastes can be transformed into biofuel (bioethanol) by making use of the fermentation process.

The highly organic nature of date palm waste makes it highly suitable for compost production which can be used to replace chemical fertilizers in date palm plantations. Thus, abundance of date palm trees in the MENA and the Mediterranean region, can catalyze the development of biomass and biofuels sector in the region.

The post Date Palm Wastes as a Biomass Resource first appeared on BioEnergy Consult.