Synthetic Biology – A Catalyst to Revolutionize Biogas Industry

Essentially a process operating by living organisms, the biogas industry is a natural target for synthetic biology. Synthetic biology combines biology and engineering to design and construct biological devices. Contrary to traditional genetic engineering that only alters an already existing DNA sequence, synthetic biology allows us to build entirely new sequences of DNA and put them to work in cells. This allows us to build novel biological devices that would never exist in nature.

Constructions and operations of devices that do not exist in nature, such as tools, vehicles, computers and the internet, have crafted modern civilization. Now, it is synthetic biology that is challenging nature’s limitations and advancing civilization to a higher level.

Generating biogas via anaerobic digestion of biomass and organic waste is one of the few proven, cost-effective, scalable biomass energy strategies. Biogas consists of mainly methane and carbon dioxide, and combustion of methane with air generates energy which can be used for many purposes such as cooking, heating, producing electricity and vehicle fuel. As a result, countless biogas plants are operating around the globe helping to clean up waste and generate energy. With more plants being built, they come in all sizes ranging from household to factory scales.

Anaerobic digestion is a process where extremely complex microbial communities degrade organic matter, such as sugars, fats and proteins, resulting in biogas as the primary end-product. Such inherent complexity makes this process very difficult to optimize. Mechanical engineers have made tremendous progress to optimize this process, but in many places it still requires government subsidies to be profitable.

Synthetic Biology and Biogas Industry

Essentially a process operating by living organisms, the biogas industry is a natural target for synthetic biology. In terms of their genetic content, organisms are classified into three natural groups, Archaea, Bacteria and Eukarya. Most microbes are Archaea and Bacteria, while humans are Eukarya.

In an anaerobic digester, many different types of Bacteria convert the complex organic matter in waste or biomass to hydrogen gas, carbon dioxide, formate and acetate. A unique group of methanogenic Archaea then produces the invaluable part of biogas, methane, by eating hydrogen and carbon dioxide, formate or acetate.

One can imagine creating a super microbe to convert the complex organic matter directly into biogas, thus making anaerobic digestion faster, more efficient and easier-to-manipulate. Making a synthetic microbial community by reprogramming key microbes may also help them work together when a tough job (i.e., eating extremely complex waste) needs to be done.

Among numerous microbes in anaerobic digester, methanogenic Archaea are one of a few microbial groups that have been extensively studied, and a number of genetic tools are available for engineering via synthetic biology. Therefore, scientists have begun to reprogram methanogenic archaea, allowing them to eat organic matter such as sugars and directly produce methane. If they succeed, they may engineer a super microbe that never existed in nature and revolutionize the biogas industry by making anaerobic digestion much simpler and more efficient.

There is also the possibility of more applications downstream. For instance, upgrading biogas by removal of carbon dioxide improves its combustibility. A super microbe could be made to upgrade biogas using hydrogen gas or even electricity to form more methane from carbon dioxide.

Conceptualized super cell that converts idealized organic matter (2CH2O) directly into biogas.

Grand Challenges

However promising, grand challenges remain when it comes to applying synthetic biology to the biogas industry. About 10,000 moving parts are needed to make an automobile, millions of parts for an airplane, and all the parts are standardized.

Similar to those engineering sectors, synthetic biology also needs many standardized genetic parts and modules to be able to create biological devices that can really revolutionize an industry. Sophisticated genetic tools are needed as well to assemble these parts and put them to work. However, few such parts, modules and tools are at disposal for engineering microbes in an anaerobic digester.

Take methanogenic Archaea for example, only three parts are available in the iGEM registry, the world largest collection of biological parts for synthetic biology. Another challenge is an apparent neglect of synthetic biology by the biogas industry. Symposiums bringing professionals from biogas industry and synthetic biology together for discussions are rare, as are major investments for promoting synthetic biology.

As a result, few research groups are developing synthetic tools and parts for the biogas industry. For example, the aforementioned three iGEM parts were all contributed by only one group, the UGA-iGEM team at the University of Georgia.

Future Perspectives

Synthetic biology is developing faster than ever, and its cost continues to fall. Thanks to prompt actions of many industrial pioneers in embracing and supporting synthetic biology, it is already starting to revolutionize a few fields.

Synthetic biology also holds great potentials to revolutionize the biogas industry. To achieve this goal, joint efforts between the biogas industry and academia must be made. The former side needs to understand what synthetic biology can achieve, while the latter side should identify which parts of the process in the biogas industry can be re-designed and optimized by synthetic biology.

Once the two sides start to work together, novel synthetic parts and tools are bound to be invented, and they will make anaerobic digestion a better process for the biogas industry.

Description of a Biogas Power Plant

A biogas plant is a decentralized energy system, which can lead to self-sufficiency in heat and power needs, and at the same time reduces environmental pollution. The components of a modern biogas (or anaerobic digestion) plant include: manure collection, anaerobic digester, effluent treatment, biogas storage, and biogas use/electricity generating equipment.

Working of a Biogas Plant

The fresh animal manure is stored in a collection tank before its processing to the homogenization tank which is equipped with a mixer to facilitate homogenization of the waste stream. The uniformly mixed waste is passed through a macerator to obtain uniform particle size of 5-10 mm and pumped into suitable-capacity anaerobic digesters where stabilization of organic waste takes place.

In anaerobic digestion, organic material is converted to biogas by a series of bacteria groups into methane and carbon dioxide. The majority of commercially operating digesters are plug flow and complete-mix reactors operating at mesophilic temperatures. The type of digester used varies with the consistency and solids content of the feedstock, with capital investment factors and with the primary purpose of digestion.

Biogas Cleanup

Biogas contain significant amount of hydrogen sulfide (H2S) gas which needs to be stripped off due to its highly corrosive nature. The removal of H2S takes place in a biological desulphurization unit in which a limited quantity of air is added to biogas in the presence of specialized aerobic bacteria which oxidizes H2S into elemental sulfur.

Utilization of Biogas

Biogas is dried and vented into a CHP unit to a generator to produce electricity and heat. The size of the CHP system depends on the amount of biogas produced daily.

Treatment of Digestate

The digested substrate is passed through screw presses for dewatering and then subjected to solar drying and conditioning to give high-quality organic fertilizer.  The press water is treated in an effluent treatment plant based on activated sludge process which consists of an aeration tank and a secondary clarifier. The treated wastewater is recycled to meet in-house plant requirements.

Monitoring of Environmental Parameters

A chemical laboratory is necessary to continuously monitor important environmental parameters such as BOD, COD, VFA, pH, ammonia, C:N ratio at different locations for efficient and proper functioning of the process.

Control System

The continuous monitoring of the biogas plant is achieved by using a remote control system such as Supervisory Control and Data Acquisition (SCADA) system. This remote system facilitates immediate feedback and adjustment, which can result in energy savings.